Purpose- To record electrical activities of individual neurons while you deliver mechanical stimulus to the attached skin. Inject florescent dyes into the neurons to visualize their morphology.
Hypothesis- Each of the areas of the neurons are specialized to each do a different specific job.
Materials- Leech tank, dissection tray, 20% ethanol, dissecting microscope, oscilloscope, micromanipulator, scalpel, probe, forceps, scissors, dissection pins, leech tongs.
Procedure-
Anesthetize the leech with 20% ethanol and cut into it using the scissors.
Use forceps to pull skin flaps open and pin down with the dissection pins.
Remove leech innards with the probe and observe ganglion
Cut out the ganglion window using scissors
Isolate one ganglion
Cut the ganglion sinus using the scalpel
Probed and identified ganglion sensory cells using the forceps to pull the skin open and the pins to keep it open.
Observe cells under dissecting microscope, using the micromanipulator to inject dye into different areas of the neuron and put under UV light to identify the different cell types. Also, use different stimuli such as a feather or forceps to see if there is a reaction of action potential in the cell that is being probed.
Results- Each of the sensory cells examined in different areas of the ganglion had different shapes with different functions. Different stimuli produced different types of responses. The atlas used helped to identify which type of cell responded to which type of stimuli in which way.
Conclusions- Different cells in the neuron have different functions based on their structure and location. Also, the different stimuli (the feather, probe and forceps) made the different types of cells respond in different ways. The different types of cells and their locations were easily identified using the different stimuli used in combination with indication from the atlas below:
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